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Hemolysin – Wikipedia, the free encyclopedia

Posted: August 4, 2015 at 2:45 pm

Hemolysins or haemolysins are lipids and proteins that cause lysis of red blood cells by destroying their cell membrane. Although the lytic activity of some microbe-derived hemolysins on red blood cells may be of great importance for nutrient acquisition, many hemolysins produced by pathogens do not cause significant destruction of red blood cells during infection. Although hemolysins are capable of doing this for red blood cells in vitro.

As mentioned above, most hemolysins are protein compounds, but others are lipids biosurfactants.[1]

Many bacteria produce hemolysins that can be detected in the laboratory. It is now believed that many clinically relevant fungi also produce hemolysins.[2] Hemolysins can be identified by their ability to lyse red blood cells in vitro.

Not only are the erythrocytes affected by hemolysins, but there are also some effects among other blood cells, such as leucocytes (white blood cells). Escherichia coli hemolysin is potentially cytotoxic to monocytes, lymphocytes and macrophages, leading them to autolysis and death.

Visualization of hemolysis (UK: haemolysis) of red blood cells in agar plates facilitates the categorization of Streptococcus.

In the next image we can see the process of hemolysis by a Streptococcus:

One way hemolysin lyses erythrocytes is by forming pores in phospholipid bilayers.[3][4] Other hemolysins lyse erythrocytes by hydrolyzing the phospholipids in the bilayer.

Due to the importance of hemolysins and the formation of pores, this part looks forward to enhance some more aspects of the process. Many hemolysins are pore-forming toxins (PFT), which are able to cause the lysis of erythrocytes, leukocytes, and platelets by producing pores on the cytoplasmic membrane.

But, in which way does this kind of protein carry out this process?

Hemolysin is normally secreted by the bacteria in a water-resoluble way. These monomers diffuse to the target cells and are attached to them by specific receivers. After this is already done, they oligomerize, creating ring-shaped heptamer complexes.[5]

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Cedric Blanpain Lab – People

Posted: August 3, 2015 at 3:47 pm

Cedric Blanpain graduated as a Medical Doctor (1995), received his PhD in Medical Sciences (2001) and was board certified in internal medicine (2002) at the Universit Libre de Bruxelles (ULB), Belgium. Cedric performed a postdoctoral training in the laboratory of Elaine Fuchs, at the Rockefeller University, New York, USA from 2002 to 2006.

Cedric Blanpain is full professor of Stem Cell and Development Biology and investigator of the WELBIO (Walloon Excellence in Life science and Biotechnology) at the IRIBHM, Universit Libre de Bruxelles (ULB). His research group is studying the mechanism regulating stem cell fate decision during embryonic development, tissue homeostasis and repair as well as the implication of stem cells during cancer initiation and growth.

Cedric Blanpain received several prestigious awards and fellowships including the career development award of the HFSP, EMBO young investigator, ERC starting grant, Outstanding Young Investigator Award of the International Society of Stem Cell Research (ISSCR) and is an EMBO member since 2012.

The Belgian FNRS, Fondation Contre Le Cancer, Fond Ithier, Region Wallone, Region Bruxelles-Capitale, the Human Science Frontier Program Organization, the EMBO young investigator program, and the European Research Council support his research.

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Autologous mesenchymal stem cell therapy for progressive …

Posted: August 3, 2015 at 3:47 pm

Nath U, Ben-Shlomo Y, Thomson RG, Morris HR, Wood NW, Lees AJ, Burn DJ: The prevalence of progressive supranuclear palsy (Steele-Richardson-Olszewski syndrome) in the UK.

Brain 2001, 124:1438-1449.

Bower JH, Maraganore DM, McDonnell SK, Rocca WA: Incidence of progressive supranuclear palsy and multiple system atrophy in Olmsted County, Minnesota, 1976 to 1990.

Neurology 1997, 49:1284-1288.

Ludolph AC, et al.: Tauopathies with parkinsonism: clinical spectrum, neuropathologic basis, biological markers and treatment options.

Eur J Neurol 2009, 16:297-309.

Litvan I, Agid Y, Calne D, Campbell G, Dubois B, Duvoisin RC, Goetz CG, Golbe LI, Grafman J, Growdon JH, Hallett M, Jankovic J, Quinn NP, Tolosa E, Zee DS: Clinical research criteria for the diagnosis of progressive supranuclear palsy (Steele-Richardson-Olszewski syndrome): report of the NINDS-SPSP international workshop.

Neurology 1996, 47:1-9.

Litvan I, Campbell G, Mangone CA, Verny M, McKee A, Chaudhuri KR, Jellinger K, Pearce RK, DOlhaberriague L: Which clinical features differentiate progressive supranuclear palsy (SteeleRichardsonOlszewski syndrome) from related disorders? A clinicopathological study.

Brain 1997, 120:65-74.

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Interactives . DNA . Genetic Engineering

Posted: August 3, 2015 at 9:41 am

Genetic engineering is the process of removing a gene from one organism and putting it into another. Often, the removed genes are put into bacteria or yeast cells so that scientists can study the gene or the protein it produces more easily. Sometimes, genes are put into a plant or an animal.

One of the first genetic engineering advances involved the hormone insulin. Diabetes, a medical condition that affects millions of people, prevents the body from producing enough insulin necessary for cells to properly absorb sugar. Diabetics used to be treated with supplementary insulin isolated from pigs or cows. Although this insulin is very similar to human insulin, it is not identical. Bovine insulin is antigenic in humans. Antibodies produced against it would gradually destroy its efficacy.

Scientists got around the problem by putting the gene for human insulin into bacteria. The bacteria's cellular machinery, which is identical to the cellular machinery of all living things, "reads" the gene, and turns it into a protein-human insulin-through a process called translation.

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Interactives . DNA . Genetic Engineering

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Glossary Index | womenshealth.gov

Posted: August 3, 2015 at 9:41 am

Find your glossary term by first letter:

a form of complementary and alternative medicine that involves inserting thin needles thorugh the skin at specific points on the body to control pain and other symptoms.

a form of complementary and alternative medicine that involves inserting thin needles thorugh the skin at specific points on the body to control pain and other symptoms.

written instructions letting others know the type of care you want if you are seriously ill or dying. These include a living will and health care power of attorney.

written instructions letting others know the type of care you want if you are seriously ill or dying. These include a living will and health care power of attorney.

disorders that involve an immune response in the body. Allergies are reactions to allergens such as plant pollen, other grasses and weeds, certain foods, rubber latex, insect bites, or certain drugs.

tiny glands in the breast that produce milk.

a brain disease that cripples the brain's nerve cells over time and destroys memory and learning. It usually starts in late middle age or old age and gets worse over time. Symptoms include loss of memory, confusion, problems in thinking, and changes in language, behavior, and personality.

clear, slightly yellowish liquid that surrounds the unborn baby (fetus) during pregnancy. It is contained in the amniotic sac.

when the amount of red blood cells or hemoglobin (the substance in the blood that carries oxygen to organs) becomes reduced, causing fatigue that can be severe.

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Glossary Index | womenshealth.gov

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Asymmetrex Plans to Report Results From Adult Stem Cell …

Posted: August 2, 2015 at 10:44 am

It is a summer of anticipation for Bostons stem cell medicine biotechnology start-up Asymmetrex. The company has key milestone studies underway for continued development of its unique portfolio of adult tissue stem cell biotechnologies for regenerative medicine and drug development.

Boston, MA (PRWEB) July 29, 2015

Asymmetrex, LLC founder and director James L. Sherley says the company is making good progress in evaluating the full range of capabilities of its unique cache of technologies for identifying, quantifying, and producing adult tissue stem cells. Adult tissue stem cells perform the day-to-day renewal and repair of normal tissues and organs. This property makes them the essential therapeutic factor in regenerative medicine treatments developed to restore diseased or injured tissues. The essential role of adult tissue stem cells in maintaining normal tissues also makes them important determinants of drug safety.

Despite the widely understood importance of adult tissue stem cells for advancing biomedicine, in the past, implementation of this knowledge has been largely unachievable because of long-standing technology barriers. In many cases, adult tissue stem cells have been difficult to identify, impossible to count, and difficult to produce in therapeutic quantities. These significant technical challenges have limited regenerative medicine treatments to only a few tissue stem cell types and essentially precluded evaluation of drug candidate effects on critical tissue stem cell types.

Says Sherley, Asymmetrex seeks to catalyze a new era of stem cell biomedicine. The company plans to achieve catalysis by reaching a tipping point for the widespread use of its patented technologies for identifying, counting, and producing adult tissue stem cells from many different human tissues. In addition, because Asymmetrexs technologies are based on universal tissue stem cell principles, they also have application for animal research and veterinary medicine.

Director Sherley notes two studies that the company is pursuing this summer that will be completed by early fall and one that will continue until next April, or longer if needed. For its development of adult tissue stem cell production technologies, Asymmetrex is conducting a series of standard pharmaceutical analyses of mature liver cell properties for cells produced from the companys expanded human liver stem cells. Asymmetrex has a patented technology for producing human liver stem cells in pound quantities. Establishing that the expanded liver stem cells can be used to make similarly large quantities of differentiated cells with mature liver functions is an important milestone. This achievement would advance the tissue stem cell expansion technology to applications for pharmaceutical drug evaluations and liver cell transplantation therapies for liver failure patients.

The second summer study is an evaluation of a new technology developed with partner AlphaSTAR Corporation. AlphaSTEM technology is a proprietary human cell culture-computer simulation technology that can estimate and monitor tissue stem cell number, viability, and function for many different human tissues. AlphaSTEM technology could be used to estimate stem cell dose and determine stem cell quality for regenerative medicine stem cell transplantation therapies. Neither of these capabilities currently exists.

A major commercialization thrust of the two partner companies is the use of AlphaSTEM technology to identify tissue stem cell-toxic drug candidates before expensive pre-clinical animal studies and clinical trials. Estimates indicate that, with widespread adoption, the technology could save the U.S. pharmaceutical industry as much as $4 billion each year by preventing expensive testing of drug candidates that would ultimately fail because of toxicity against tissue stem cells. The current study is an evaluation of a panel of known stem cell-activating, stem cell-toxic, and neutral agents designed to begin estimating the predictive power of AlphaSTEM technology.

The third, year-long or greater, study might also yield new developments as soon as the end of the summer. Asymmetrexs most recent patent (U.S. 9,081,008) is for a unique set of biomarkers with sufficient specificity to enable counting of adult tissue stem cells for the first time. In April, the company launched a crowdsourcing campaign to engage investigators worldwide in evaluating its most specific biomarker called H2A.Z asymmetry for counting adult tissue stem cells that are the focus of their research or clinical studies. H2A.Z is a chromosome factor involved in selective gene regulation. In functioning adult tissue stem cells reported to date, it displays a unique pattern that can be used to identify and count tissue stem cells.

Director Sherley is scheduled to discuss the cellular basis of Asymmetrexs biomarkers for counting tissue stem cells and give an update on the progress of the H2A.Z asymmetry crowdsourcing campaign at the 8th International Epigenetics, Stem Cells, Sequencing & SNiPs-2015 Meeting from August 24-25 in Boston. The outcomes from the two summer studies will be reported at BioPharm America 2015, September 15-17, in Boston.

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Human Embryonic Stem Cell Market Share, Growth, Trends …

Posted: August 2, 2015 at 10:43 am

This article was originally distributed via SproutNews. SproutNews, WorldNow and this Site make no warranties or representations in connection therewith.

GrandViewResearch.com has announced the addition of Global Human Embryonic Stem Cell Market Analysis And Segment Forecasts To 2020 Market Research report to their Database

The global human embryonic stem cell market is anticipated to gain lucrative growth over the forecast period owing to rising incidences of genetic, blood and immunity associated disorders. Increasing geriatric population base is also a high impact rendering driver of this market. Moreover, rising preference by physicians owing to overcoming limitations in current therapies including tissue damage and frequent disease relapse cases are also expected to propel the growth of this market over the next seven years. Furthermore, technological advancements and rising usage in various medical applications such as cellular transplantation therapy and developmental biology are expected to boost demand for human embryonic stem cell in near future.

Full research report on Global Human Embryonic Stem Cell Market with detailed figures and charts available at:http://www.grandviewresearch.com/industry-analysis/human-embryonic-stem-cell-market

The global human embryonic stem cell market is segregated on the basis of application into tissue engineering, toxicology testing, regenerative medicine and stem cell biology research. As of 2014, stem cell biology research held the largest share of the market as a consequence of increasing need of early screening and treatment of genetic disorders. Tissue engineering is anticipated to be the fastest growing segment owing to associated benefits including high cost efficiency and increased stability of the repaired tissues thereby driving the demand for market over the forecast period.

Get more information on Global Human Embryonic Stem Cell Market or request for TOC of this research report at:http://www.grandviewresearch.com/industry-analysis/human-embryonic-stem-cell-market/request-toc

North America held the largest market share in 2014, owing to contributing factors such as rising incidences of genetic, cardiac and neurological disorders. Rising geriatric population base, increased awareness, high disposable income and availability of sophisticated healthcare infrastructure are also a high impact rendering drivers for this region. Asia Pacific is anticipated to be the fastest growing region over the forecast period as a consequence of increasing investments and awareness pertaining to new therapies for chronic diseases. Moreover, increasing contract research outsourcing and rising disposable income are anticipated to propel growth potential for this region over the forecast period.

The major market players include Vitrolife AB, Celgene Corporation Inc., Biotime Inc., Geron, Brainstorm Cell Therapeutics, Osiris Therapeutics, California Stem Cell Inc., NovoCell, Cell Dynamics International, Cellartis AB and Angel Biotechnology. These companies employ strategies including collaborations, geographic diversification and extensive clinical trials in order to capitalize their market share. Moreover, promoting medical tourism and reducing marketing expenditure are expected to expand their market share over the forecast period. For instance, development of innovative products such as disposable cell culture devices, tridimensional bioreactors and nanosensors are expected to boost the demand for the market. These advancements result in facilitating longer preservation of stem cells and real time monitoring of cells that are anticipated to drive the demand for the market over the forecast period.

View more reports of this category by Grand View Research at:http://www.grandviewresearch.com/industry/biotechnology

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Orthopedic and Sports Performance Institute in Gilbert AZ …

Posted: August 2, 2015 at 10:43 am

East Valley Stem Cell Doctors during OSPI (844) GET-STEM

This is changing a approach orthopedic and pain government caring is charity in a US. Now a Board Certified doctors can indeed assistance correct patients conditions outward of a handling room!

Gilbert, AZ (PRWEB) July 13, 2015

Orthopedic and Sports Performance Institute in Gilbert AZ, famous as OSPI, is now an R3 Stem Cell Center of Excellence. The Board Certified East Valley orthopedic doctors during a use are charity insubordinate branch dungeon procedures for those pang from degenerative and rheumatoid arthritis, sports injuries and tendonitis/ligament injuries. New patients are now being scheduled for consultations and procedures by job (844) GET-STEM.

Stem dungeon procedures have been mainstream for years now, and continued investigate is display their regenerative energy during assisting patients equivocate or check a need for hip and knee deputy surgeries. Pain service is typically estimable with a outpatient procedures, and duty is softened dramatically.

In addition, branch dungeon therapy can assistance patients grasp poignant service with conditions such as rotator slap tendonitis/tears, Achilles tendonitis, sports injuries and dire injuries. Unlike normal treatments with cortisone injections, a stem dungeon treatments have a capability to reanimate repairs and correct damage. Athletes can get behind on a margin quickly, and patients with injuries mostly equivocate potentially unsure surgeries.

OSPI CEO Derek Price stated, This is changing a approach orthopedic and pain government caring is charity in a US. Now a Board Certified doctors can indeed assistance correct patients conditions outward of a handling room!

Several opposite forms of branch dungeon procedures are charity for patients, with a East Valley plcae saying patients from Chandler, Gilbert, Queen Creek, Mesa, San Tan, Casa Grande, Maricopa AZ and more.

R3 Stem Cell is a national network of practices charity regenerative medicine procedures. Only tip tier practices are invited into a Network, with Board Certified providers who are rarely regarded in their fields. OSPI is a tip notch, singular use in Gilbert with Board Certified providers in orthopedics, pain management, podiatry, earthy therapy and more.

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Testosterone for Men Testosterone Therapy for Men

Posted: August 2, 2015 at 10:42 am

The androgen group of hormones includes any substance that assists in the development and manages the appearance of male characteristics. The principal hormone in this group is testosterone, an anabolic steroid. Anabolic steroids promote the retention of protein in the body, contribute to muscle mass, bone strength, bone density and more. Testosterone, which stimulates male puberty, is derived from cholesterol and produced in the testes.

Testosteroneserves several purposes in the male body including the growth and development of sexual characteristics. Thetestosterone hormonealso deepens the voice during puberty, stimulates the growth of facial and body hair, increases sexual drive, and is responsible for sperm production. Furthermore, testosterone contributes to liver function, muscle mass and strength, the ability to produce erections and sexual stamina. While women also possess testosterone, it is in much smaller levels. Testosterone is the main male hormone that differentiates men from women.

To put it simply,andropause(male menopause) is caused by low testosterone. As men age, their testosterone production decreases by about 2% a year causing a shortage of this very necessary hormone. The testosterone shortage paired with increasing levels of the Sex Binding Hormone Globulin (SHBG) which inhibits a substantial portion of remaining testosterone from working, causes the andropause symptoms (i.e.erectile problems, hair loss, weight gain,low sexual drive) because there is not enough testosterone to perform all of the functions that the body relies on it for. Hair loss in particular is caused by a hormone created from testosterone, dihydrotestosterone or DHT, which when bound to a particular receptor in male hair follicles, causes thinning hair or hair loss.

As mentioned, andropause is the result of diminished testosterone levels in the male body. It is responsible for thosesymptoms of andropauseand can actually cause:

Testosterone therapy or testosterone replacement can return the male body to producing normal testosterone levels.

While uncommon, there are cases of men possessing an excess of testosterone, this is most clearly seen in steroid use among athletes. Symptoms of high testosterone include but are not limited to:

State-of-the-art testing by BodyLogicMD can inform you if you possess too much testosterone, at which point, yourbioidentical hormone doctorwill prescribe a treatment plan to rectify your testosterone levels.

The BodyLogicMD process starts with simple yet thorough testing to identify not only the levels of testosterone in your body, but also a series of other hormones that may be causing similar symptoms and problems. If you are low on testosterone, your BodyLogicMD affiliated physician will prescribecustomized testosterone hormone therapyfor bioidentical testosterone replacement. Common treatments include testosterone creams or under the skin pellets. Furthermore, an individualized nutrition, supplement and fitness program will be suggested to help you maintain your new hormone balance. Getting older is inevitable, aging is not. Hair loss, low sex drive, erectile problems and a loss of muscle mass may be avoidable with the help ofbioidentical hormone replacement therapywith BodyLogicMD.

For the most part, testosterone replacement therapy is safe when used properly. However, like any medication or therapy, there are some side effects from testosterone replacement therapy that may occur. Side effects of testosterone therapy include:

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The Miami Stem Cell Treatment Center Announces an Adult …

Posted: August 1, 2015 at 3:49 pm

Upcoming Adult Stem Cell Lecture on Thursday, August 6, 2015, in Orlando at 4:00 pm

Orlando, Florida (PRWEB) July 30, 2015

The Miami Stem Cell Treatment Center announces a free public seminar on the use of adult stem cells for various degenerative and inflammatory conditions. They will be provided by Dr. Thomas A. Gionis, Surgeon-in-Chief, and Dr. Nia Smyrniotis, Medical Director.

The seminar will be held on Thursday, August 6, 2015, at 4:00 pm at our Orlando office, 100 North Dean Road, 2nd Floor, Orlando, FL 32825. Please RSVP at (561) 331-2999, all events are by reservation only.

The Miami Stem Cell Treatment Center (Miami; Boca Raton; Orlando; The Villages; Sarasota, Florida), along with sister affiliates, the Irvine Stem Cell Treatment Center (Irvine; Westlake Village; Sacramento, California) and the Manhattan Regenerative Medicine Medical Group (Manhattan, New York), abide by approved investigational protocols using adult adipose derived stem cells (ADSCs) which can be deployed to improve patients quality of life for a number of chronic, degenerative and inflammatory conditions and diseases. ADSCs are taken from the patients own adipose (fat) tissue (found within a cellular mixture called stromal vascular fraction (SVF)). ADSCs are exceptionally abundant in adipose tissue. The adipose tissue is obtained from the patient during a 15 minute mini-liposuction performed under local anesthesia in the doctors office. SVF is a protein-rich solution containing mononuclear cell lines (predominantly adult autologous mesenchymal stem cells), macrophage cells, endothelial cells, red blood cells, and important Growth Factors that facilitate the stem cell process and promote their activity.

ADSCs are the bodys natural healing cells - they are recruited by chemical signals emitted by damaged tissues to repair and regenerate the bodys injured cells. The Miami Stem Cell Treatment Center only uses Adult Autologous Stem Cells from a persons own fat No embryonic stem cells are used; and No bone marrow stem cells are used. Current areas of study include: Emphysema, COPD, Asthma, Heart Failure, Heart Attack, Parkinsons Disease, Stroke, Traumatic Brain Injury, Lou Gehrigs Disease, Multiple Sclerosis, Lupus, Rheumatoid Arthritis, Crohns Disease, Muscular Dystrophy, Inflammatory Myopathies, and degenerative orthopedic joint conditions (Knee, Shoulder, Hip, Spine). For more information, or if someone thinks they may be a candidate for one of the adult stem cell protocols offered by the Miami Stem Cell Treatment Center, they may contact Dr. Gionis or Dr. Smyrniotis directly at (561) 331-2999, or see a complete list of the Centers study areas at: MiamiStemCellsUSA.com.

Also, you can listen and call into our radio show, The Stem Cell Show, hosted by Dr. Gionis worldwide on KABC.com ("Listen Live" at 7pm EST) or the KABC app available on the App Store or Google Play.

About the Miami Stem Cell Treatment Center: The Miami Stem Cell Treatment Center, along with sister affiliates, the Irvine Stem Cell Treatment Center and the Manhattan Regenerative Medicine Medical Group, is an affiliate of the California Stem Cell Treatment Center / Cell Surgical Network (CSN); we are located in Miami, Boca Raton, Orlando, The Villages, and Sarasota, Florida. We provide care for people suffering from diseases that may be alleviated by access to adult stem cell based regenerative treatment. We utilize a fat transfer surgical technology to isolate and implant the patients own stem cells from a small quantity of fat harvested by a mini-liposuction on the same day. The investigational protocols utilized by the Miami Stem Cell Treatment Center have been reviewed and approved by an IRB (Institutional Review Board) which is registered with the U.S. Department of Health, Office of Human Research Protection (OHRP); and our studies are registered with Clinicaltrials.gov, a service of the U.S. National Institutes of Health (NIH). For more information, visit our websites: MiamiStemCellsUSA.com, IrvineStemCellsUSA.com, NYStemCellsUSA.com, or TheStemCellShow.com.

For the original version on PRWeb visit: http://www.prweb.com/releases/2015/07/prweb12861292.htm

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