Brain Tumor Stem Cell Lab – Massachusetts General Hospital …

Research Projects

Characterizing glioblastoma stem cells

One of the main research focuses of the Brain Tumor Stem Cell Lab (Wakimoto Lab) is to characterize glioblastoma stem cells isolated from patient tumors. We are extensively establishing glioblastoma stem cells that grow in culture and testing their ability to form experimental brain tumors in mice. Our research shows that the capability of glioblastoma stem cells to migrate and invade through the brain varies greatly between patients. We are interested in identifying the molecular mechanisms that control tumor invasiveness. These studies are being conducted in close collaboration with the laboratories of Drs. Martuza, Rabkin, Curry, Cahill, Batchelor and Chi at Massachusetts General Hospital.

Therapeutic development

Glioblastoma stem cells are considered resistant to therapy such as chemotherapeutic drugs, but our research suggests that this is not always the case. Using glioblastoma stem cells as a unique model system that preserves patient-specific disease characteristics, we are evaluating the efficacy of therapeutic agents that include oncolytic herpes simplex virus -1 (in collaboration with the Martuza and Rabkin laboratories) and novel molecular targeting agents (in collaboration with the Batchelor and Chi Laboratories), and trying to discover molecular signatures of cells that help predict effectiveness for such treatment. Working closely with the Shah Laboratory of Radiology at Mass General, we are conducting research that harnesses normal stem cells to deliver therapeutic viruses to glioblastoma in the brain.

Glioblastoma recurrence

Cancer recurrence after surgery, radiation and chemotherapy is a serious issue that makes the management of glioblastoma difficult. We are trying to understand the role glioblastoma stem cells play in the relapse of the tumor. Our approach to this task is to establish glioblastoma stem cells from tumors both before and after therapy, and compare the two stem cells through analysis of their molecular and biological characteristics.

Collaboration

Taking advantage of the rich scientific environment at Mass General, the Brain Tumor Stem Cell Lab (Wakimoto Lab) works closely with the laboratories of Drs. Martuza, Rabkin, Curry, Cahill, Batchelor, Chi, Bernstein, Mohapatra, Iafrate and Shah. We are part of the Molecular Neurosurgery Lab and the Brain Tumor Research Center.

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Brain Tumor Stem Cell Lab - Massachusetts General Hospital ...