Epigenetics: DNA Methylation, Chromatin Modification …

Epigenetics describes changes that are stable, but potentially reversible alterations in gene expression, that occur without permanent changes in DNA sequence and can still be passed on from generation to generation. Epigenetically controlled genes are activated or repressed without any change in DNA. Three central epigenetic mechanisms that play an essential role in gene regulation have been extensively studied by researches, including DNA methylation, histone modification, and RNA regulation. Our combined comprehensive Epigenetics portfolio offer high quality products to perform the techniques being utilized to study all the three central epigenetic mechanisms.

Quantitative detection of histone modifications is important to a better understanding of epigenetic regulation of cellular processes in normal or cancer tissues. The most widely used techniques to study how histone modifications and other DNA binding proteins, such as transcription factors, influence gene expression is called chromatin immunoprecipitation (ChIP) combined with qualitative polymerase chain reaction (qPCR). ChIP involves chemically crosslinking proteins to DNA sequences, which is followed by immunoprecipitation of the crosslinked complexes by using antibodies and beads to pull down the modified histone or other proteins of interest. Below are some selected products for your research in histone modification. The most commonly studied and best understood histone modifications are acetylation, phosphorylation, methylation, and ubiquitination. Histone modifications regulate DNA transcription, repair, recombination, and replication, and can alter local chromatin architecture. We offer a wide range of kits for analyzing complex histone modifications patterns.

Histone Modification Assays

Methylated DNA Enrichment, Isolation, Visualization and Quantification

Whole genome amplification (WGA)

Transcriptome Amplification

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Epigenetics: DNA Methylation, Chromatin Modification ...