Category Archives: Florida Stem Cells

23 hours ago Fig. 1: Semiconductor image sensor

Professor Kazuaki Sawada and Dr. Takigawa of the National Center for Geriatrics and Gerontology and colleagues at Toyohashi University of Technology have established an easy to use, low-cost, rapid, and high sensitivity semiconductor-imaging based medical diagnostic biosensing system for analyzing blood and urine for early diagnosis of ailments including diabetes and Alzheimer's disease.

The new biosensing technology consists of a semiconductor image sensor ( 'charge coupled device' developed by Toyohashi University of Technology) that is sensitive to extremely small changes in electric potential, and microbeads on which antigen-antibody reactions take place. This technology will enable monitoring and diagnosis of diseases for which specific markers are known using very small volumes of blood or urine. Specifically, this technology has detected amiloid beta-peptide, an agent responsible for Alzheimer's disease.

Contracting a disease leads to expression of proteins specific to the diseases in the blood. This new technology is used for early diagnosis of diseases by using this specific protein as the antigen and a marker that captures the protein as the antibody and checking their antigen-antibody reaction. Conventional protocols used to monitor antibody-antigen reactions employ fluorescent probes and detection of fluorescence with microscopic cameras. This process is time consuming because of the necessity to measure fluorescence from the probes and cannot be used to detect low concentrations of antigens when the fluorescence intensity is too low to detect optically.

With this technology, an antigen-antibody reaction is used as in conventional methods, but fluorescence is not measured. Instead, this method employs a semiconductor image sensor to detect minute changes in electric potential generated during an antigen-antibody reaction.

The semiconductor image sensor [Fig. 1] consists of 128 128 pixels that independently sense minute changes in electric potential. The detection sensitivity of antigen-antibody reactions was significantly increased by using microbeads [Fig. 2]. The figures of merit of this technology are given in Table 1. Multiple diseases can also be simultaneously diagnosed by placing different antibodies on different sensing pixels out of a total of 16,384 pixels (128128).

Implementation of the technology will be tested for daily control of lifestyle diseases such as diabetes and in future the technology will be expanded for the early diagnosis of Alzheimer's and Parkinson' diseases.

Explore further: New technique could benefit Alzheimer's diagnosis

More information: Akiteru Kono et al, "Label free bio image sensor for real time monitoring of potassium ion released from hippocampal slices," Sensors and Actuators B 201, 439443, (2014). dx.doi.org/10.1016/j.snb.2014.04.019

A new recombinant antibody can detect and isolate mesenchymal stem cells (MSCs), a nonembryonic source of stem cells with promising applications in tissue engineering, blood stem cell transplantation, and ...

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Image sensor for analysis of blood samples for early diagnosis of diabetes and Alzheimer's disease

PUBLIC RELEASE DATE:

3-Sep-2014

Contact: Robert Miranda cogcomm@aol.com Cell Transplantation Center of Excellence for Aging and Brain Repair

Putnam Valley, NY. (Sept. 3, 2014) A team of researchers from Korea and Canada have found that transplantation of B10 cells (a stable immortalized human bone marrow derived mesenchymal stem cell line; B10 hMSC) directly into the bladder wall of mice modeled with spinal cord injury (SCI) helped inhibit the development of bladder fibrosis and improved bladder function by promoting the growth of smooth muscle cells in the bladder.

The study will be published in a future issue of Cell Transplantation and is currently freely available on-line as an unedited early e-pub at: http://www.ingentaconnect.com/content/cog/ct/pre-prints/content-CT-1227_Lee.

Spinal cord injury (SCI) can cause severe lower urinary tract dysfunction and conditions such as overactive bladder, urinary retention and increased bladder thickness and fibrosis. HMSCs, multipotent cells that can differentiate into a variety of cell types, including bone cells, cartilage cells, and fat cells, have been transplanted into injured spinal cords to help patients regain motor function.

In this study, mice receiving the B10 hMSCs injected directly into the bladder wall experienced improved bladder function while an untreated control group did not.

"Human MSCs can secrete growth factors," said study co-author Seung U. Kim of the Division of Neurology at the University of British Columbia Hospital, Vancouver, Canada. "In a previous study, we showed that B 10 cells secrete various growth factors including hepatocyte growth factor (HGF) and that HGF inhibits collagen deposits in bladder outlet obstructions in rats more than hMSCs alone. In this study, the SCI control group that did not receive B10 cells showed degenerated spinal neurons and did not recover. The B10-injected group appeared to have regenerated bladder smooth muscle cells."

Four weeks after the onset of SCI, the treatment group received the B10 cells transplanted directly into the bladder wall. To track the transplanted B10 cells via magnetic resonance imaging (MRI), the researchers labeled them with fluorescent magnetic particles.

"HGF plays an essential role in tissue regeneration and angiogenesis and acts as a potent antifibrotic agent," explained Kim.

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Transplanted stem cells help prevent bladder fibrosis after spinal cord injury

By: Alexa Williams NewsWest 9

"Whenever I ride it gets everything out of my head,'' Gage Klein, 11-year-old fighting an incurable disease, said.

For just being a sixth grader, Gage has a lot on his mind. Two years ago, he was in a motocross accident which turned into a life saving event.

"He flipped his dirt bike and it knocked him unconscious. As precaution I took him to the emergency room where we found out that the doctors were just in awe that this baby was still alive," Barbara Klein, Gage's mother, said.

Turns out, Gage has a rare incurable disease called Fanconi Anemia.

"It's bone marrow failure where his body doesn't allow his bone marrow to produce blood cell platelets. He's in bone marrow failure," Barbara said.

Gage will have to have a bone marrow transplant within the next six months to prolong his life.Unfortunately, for the Klein family, tragedy has already struck once. In 2007, they lost a son when he was just seven months old to SIDS. However, they saved the baby's stem cells at birth which gave them hope for Gage.

"They pulled the stem cells from the baby to see if they would match Gage and they were a perfect match, but unfortunately we cannot use those because the baby has passed and we need fresh blood, we need fresh skin cells," Barbara said.

The family has still not found a match.

"We haven't found a donor, we're still looking for a donor," Barbara said.

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Andrews Boy Fights Incurable Disease

What Is Stem Cell Therapy

Stem cells have the remarkable potential to develop into many different cell types in the body during early life and growth. In addition, in many tissues they serve as a sort of internal repair system, dividing essentially without limit to replenish other cells as long as the person or animal is still alive. In living tissue, the main purpose of adult stem cells is to use cell division to repair and replace dying cells in order to regenerate damaged tissue. As we age our stem cells become less active, less numerous, or both.

Stem cells can be stimulated with concentrated growth factors in platelet rich plasma (PRP) from a patients own blood. Stem cells are the engineer or builder cells that can help repair damaged structures. Each year millions of Americans suffer from musculoskeletal and arthritis pain. While some people get better over time, a significant number progress to chronic pain.

The causes of musculoskeletal pain are numerous. Cartilage in joints can be damaged from trauma, an imbalance in the supporting structures causing compensation and accelerated overuse, or normal degenerative wear and tear with age. Muscles and tendons can be damaged in similar ways. Acute injuries can damage tissue, which can lead to scar tissue and chronic weakness of that body part. Surgery can become complicated or not effective. The general indication for PRP and stem cell procedures is for injury of connective tissue or cartilage which has not responded to the usual pattern of medical intervention.

The benefits of regenerative stem cell and PRP procedures are primarily to reduce pain and increase function. Candidates also seek alternative treatments to surgery, which is not always successful, and has its own inherent risks, costs, and recuperation time. Patients are able to feel younger, continue to function and perform activities that they are used to, and not worry about a long recuperation or rehabilitation period.

Cortisone or steroid injections are sometimes useful, but chronic and repetitive cortisone injections can actually weaken and damage joint structures. The use of opiate and/or anti-inflammatory medications is usually reduced because of decreased pain. The inherent risks, costs, and complications with long term use of these medications is then decreased or removed.

Our procedures are done on an outpatient basis under fluoroscopic or ultrasound guidance. There is usually only minimal discomfort that is easily controlled with local anesthesia. There are no astronomical hospital or surgical bills, scheduling can be done within a few days and the procedure usually lasts an hour and patients return home to normal light duty activities immediately.

Exercise and/or physical therapy should usually start within a week. Every patient is different so consult with Dr. Alea for the exact instructions. The general response rate is that 85% of patients receive significant(at least 50%) pain relief. Subsequent hyaluronic acid joint lubricant treatments such as Hyalgan, Orthovisc, Synvisc, etc are more effective after stem cells are active inside the joints. Stem cells and PRP have also been shown to strengthen joints or ligaments intraoperatively and postoperatively by decreasing the amount of scar tissue that forms after surgery.

There is no allergy or rejection because the stem cells and PRP are from the patients own body. No negative long-term effects to this procedure have been reported. Dr. Alea does not harvest embryonic stem cells and is therefore not related to any controversial debates or other risks. Stem Cell procedures are not FDA approved.

However, our procedure is compliant within the guidelines listed in the FDA Code of Federal Regulations 21 Part 1271. Modern medicine in the US and throughout the world has been using stem cell transplant techniques such as bone marrow transplants to help treat patients with diseases such as leukemia, aplastic anemia, and certain inherited blood conditions for over 40 years. Bone marrow transplants are also not FDA approved, but they are also in compliance with CF21 Part 1271(121.15.b).

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South Florida Stem Cell Therapy Center

Fort Lauderdale, FL (PRWEB) November 14, 2012

MD Stem Cells reports the first treatment of an eye patient in Florida using the innovative stem cell injection technique they helped develop.

"We have helped a number of eye patients utilize Bone Marrow Derived Stem Cells or BMSC for retinal and optic nerve diseases and have observed macular degeneration, myopic degeneration, hereditary retinopathies, optic nerve and glaucoma patients, among others, have visual improvements following treatment. Our retinal surgeon has used both subretinal surgery and different injection techniques in the past for the placement of BMSC in ocular disease. We collaboratively developed a dual injection technique that we believe takes advantage of the recently identified active vascular expulsion mechanism of cell transmigration and are very excited about our ability to deliver the BMSC to the RPE, retina and optic nerve. Dr Levy mentioned that the new technique has already been used by the retinal surgeon for a number of patients in Europe and after careful preparation is now being performed in the United States.

It was a worthwhile effort to arrange for the board certified physicians and operating rooms for the new procedure to be performed in the United States. We are extremely pleased to see this high level of adult stem cell treatment come to Florida.

The Europeans have had extensive experience using BMSC in a number of different diseases and believe their effectiveness to be significantly superior to adipose derived stem cells. It is their preferred source of adult stem cells. Certainly patients with ophthalmology disease have been treated with BMSC successfully.

The new dual injection approach appears to be resulting in a faster and more vigorous response for the eye patients exclaimed Dr Levy. The retinal surgeon has been surprised regarding the tissue regrowth and rapidity of visual improvement in several patients.

With respect to BMSC, better outcomes may depend on how well the health and viability of the stem cells is maintained as well as the quality of the surgery in placing the cells. With the MD Stem Cell providers, the autologous BMSC come directly from the patient themselves, undergo minimal manipulation during the isolation process and never leave the operating room so they can be reinjected almost immediately.

This delicate approach, coupled with the precise dual placement our experienced retinal surgeon provides, may be the explanation for the results we are seeing. Most ophthalmology patients will have a stem cell procedure only once and it makes sense to choose an approach that will give the best opportunity for improvement, opined Dr Levy.

Dr Levy indicated this particular patient suffered from optic nerve disease and that other scheduled patients will include AMD or macular degeneration, myopic degeneration and other diseases of the retina and optic nerve that can be expected to potentially benefit. We believe strongly that regenerative medicine is evolving and will expand in many directions over the next few years. We are excited to help bring access to this advanced stem cell technology to the USA for ophthalmology.

MD Stem Cells is a practice management and development organization working closely with adult stem cell providers globally. Inquires may be made directly to stevenlevy(at)mdstemcells(dot)com or visit our website http://www.mdstemcells.com.

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MD Stem Cells Announces First Eye Patient Treated With ...

Welcome...

Every day, lives are changed as a result of the South Florida Bone Marrow/Stem Cell Transplant Institute ("The Institute").

Located at Bethesda Health City in Boynton Beach, Florida, the Institute offers a full range of outpatient treatments for many hematologic cancers including: Non-Hodgkins Lymphoma, Hodgkins Lymphoma, Leukemia, and Multiple Myeloma. There are also other treatments such as immunotherapy for cancers.

Under the direction of Dr. Dipnarine Maharaj, our patients are cared for by a team of highly-skilled professionals with extensive training and experience in treating cancers that react favorably to stem cell transplants. This specialized approach helps patients whose cancer has shown little or no response to other methods of treatment.

Characterized by a drive for excellence, the Institutes programs are dedicated to constantly improving the quality of care for our patients. Please take a moment to browse our website. If you would like more information on the institute or the bone marrow transplant procedure, please call561-752-5522.

Dr. Maharaj was awarded the April 2010 Physician of the month at Bethesda Memorial Hospital!

The South Florida Bone Marrow Stem Cell Transplant Institute is a accredited by the JOINT COMMISION ON ACCREDITATION OF HEALTH CARE ORGANIZATIONS (JCAHO).

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Bone Marrow Stem Cell Transplant For Myeloma, Hodgkins ...

Stem cell surgery may be the perfect treatment for those who are suffering from a bulging disc, continuous pain due to arthritis or other joint problems, those with a partially torn muscle, ligament or tendon as well as individuals who have chronic bursitis or avascular necrosis of the bone. The Institute of Regenerative and Molecular Orthopedics located in Boca Raton, Florida has now expanded their services throughout South Florida including the Miami area. Although stem cell surgical procedures are relatively new, Dr. Purita who heads this practice, and his staff, are well experienced at doing stem cell surgery, having literally done thousands of them.

Everyone has stem cells. Mesenchymal stem cells function to repair damaged tissue within the body. When injuries occur, the blood supply to the injury site can become impaired, preventing these necessary stem cells from reaching the damaged tissue, thus preventing proper healing. Because the injury site does not receive proper oxygen supply via blood, the body does not sense that there is an injury. Therefore healing cannot take place. By using stem cell surgery, the injury site receives the needed stem cells for proper healing. This is done by collecting bone marrow from the pelvic area of the patient using a small needle. Discomfort is minimal as anesthetic agents are used. The collected bone marrow is then placed in a spinning device that separates the stem cells from the bone marrow. Stem cells are then injected into the injury site, stimulating damaged cells to repair themselves.

Most patients who undergo stem cell orthopedic surgery experience significant improvements in their symptoms within 2 to 3 months, sometimes eliminating the need for surgery or long-term medication use. Full function of the treated body part often returns and pain is eliminated. This treatment can offer individuals who have been constantly suffering from orthopedic injuries or disorders to finally get the relief they deserve. Depending on the condition, repeated injections may be needed each month for about 3 to 6 months for full healing. For those who live in or around Miami, the Miami Stem Cell Surgery procedure may just be the answer you have been looking for.

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Miami Stem Cell Surgery | Molecular Orthopaedics | Florida ...

A human foot & ankle is a strong, mechanical structure that contain 26 bones, 33 joints, and more than 100 muscles, tendons & ligaments.

The 52 bones in your feet make up one quarter of all the bones in your body. When they are out of alignment, so is the rest of your body.

There are approximately 250,000 sweat glands in a pair of feet, and they excrete as much as half a pint of moisture each day.

The soles of your feet contain more sweat glands and sensory nerve endings per square centimeter than any other part of the body.

The American Podiatry Medical Association says buying shoes is best done during the afternoon. Your feet tend to swell a little during the day, and its best to buy shoes that fit then.

Women are 4 times more likely to have foot problems than men are, mostly due to footwear.

A 2-inch high heel can increase the load on the forefoot by 75%.

Many people have one foot larger than the other, so its best to fit the larger one while standing.

The American Podiatric Medical Association states the average person takes 8,000 to 10,000 steps a day, which add up to 115,000 miles in a lifetime more than 4 times the circumference of the globe.

During an average day of walking, the total forces on your feet can total hundreds of tons, equivalent to an average of a fully loaded cement truck.

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Stem Cells Florida | Stem Cell Therapy in Miami Beach By ...

erectile dysfunction treatment, type 2 diabetes treatment, arthritis cure, cure for arthritis, therapy for autism, Vision loss therapy, hair loss treatment, preventing hair loss, Pumonary disease therapy, Kidney diseases

CellTherapyFlorida U.S. Program and PRP Therapy are now being applied towards painful, injured and inflammatory conditions facilitating healing of muscle, tendons, ligaments, articular and meniscal injuries.

Loss of Hair Your own stem cells from a small area of adipose (fat) tissue can be isolated and activated. Together with a PRP and growth factors from a small sample of blood, it can be locally injected into the scalp for male and female pattern hair loss treatment.

A single treatment of Stem Cells can be of a long-term benefit. Other therapies and drugs are an hours-to-days alternative!

The utilization of insulin in the conventional treatment of diabetes mellitus is only a "symptomatic" approach, and curing diabetes involves a great deal more.

Due to the fact most of the diseases that lead to loss of vision do so as a result of abnormal vasculature and/or nerve degeneration, the use of stem cells to stabilize or prevent visual loss holds great promise.

Autism is characterized by abnormalities in social interaction, impaired verbal and nonverbal communication, and repetitive, obsessive behavior.

Regenerative cellular therapy aims for the return of damaged lung(s) to a more functional state through the use of autologous adult stem cells. Promising results have been reported in patients with lung diseases receiving this type of regenerative therapy.

Chronic kidney disease means progressive loss of the kidney function that leads to end stage kidney disease (ESKD). End stage kidney disease is the complete or almost complete kidney function failure. This condition takes place when kidneys lose their ability to maintain the day to day level of function.

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Stem Cell Treatment in Miami Florida - Call Now 1 888 545 4333