Category Archives: Arizona Stem Cells

By Nuala Moran Staff Writer

BOSTON The triumph of the Massachusetts Institute of Technology Broad Institute and its spinout company Editas Medicine Inc. in the case of CRISPR/Cas9 gene editing patents could impede the development of human therapies based on the technology, according to one of the leading researchers in the field.

George Church, professor of genetics at Harvard Medical School, said, "It definitely is an issue" if Editas maintains exclusivity in applying patents on using CRISPR/Cas9 to edit eukaryote genes. That would limit the freedom to operate of Editas' direct competitors, Intellia Therapeutics Inc. and Crispr Therapeutics AG.

Church added that even if all three companies are equally involved in translation, "that is not enough to handle all the benefits to come out of this. I would love to see more companies," he told AAAS attendees.

Church could be seen as having something of an axe to grind because he published a paper in the same issue of Science as Feng Zhang of the Broad Institute, on using CRISPR/Cas9 in human cells. It is that research of Zhang's on which the disputed patent hangs.

The decision on the high-profile CRISPR/Cas9 patents came a day after the Committee on Human Gene Editing of the National Academies of Sciences and Medicine issued a report concluding that clinical trials involving genome editing in gametes or early embryos could be permitted in the future for serious diseases or disabilities, under stringent oversight. (See BioWorld Today, Feb. 15, 2017, and Feb. 16, 2017.)

Church was one of the contributors to the international summit on gene editing held in Washington in December 2015 that led to the writing of the report. He said the report's proposals amounted to "a change in the red lines we are drawing." When talking about altering the inherited germline and the somatic cells of adults, "the line is now drawn on the seriousness of the disease," he said.

While the red line in germline modification is shifted, the line between using gene editing to cure disease and to enhance human traits becomes softer, Church suggested. For example, gene editing somatic cells to increase muscular strength in patients with muscular dystrophy could tip over into giving subjects greater than average strength.

Similarly, a somatic gene editing to improve cognition as a "cure" for Alzheimer's disease could result in patients having enhanced cognitive abilities.

Such modifications are far from becoming reality, but Church said other traits are more amenable to change. One example would be somatic modification to promote endogenous production of human growth hormone, which could be viewed both as a therapy and as a means of enhancement.

The Committee on Human Gene Editing was convened to try to get ahead of the curve in establishing regulations, before those types of modifications become feasible, as Richard Hynes, professor of cancer at MIT and co-chair of the committee, explained.

While he agreed it is difficult to draw the line between the use of somatic gene editing for treatment of disease and for enhancement, he said he firmly believes enhancement should be banned for now. "We should work out the risk/benefit for therapies first. It will take time to understand the risks. With enhancement, the benefits are debatable, but the risks are the same," Hynes said.

The concern is that, as with stem cell therapies, genetic enhancement through somatic gene editing will be on offer in rogue clinics and in countries where there is limited oversight.

Given that, one of the aims of the committee was to set out core principles that would be broadly influential and could be adopted as the basis for promulgating national laws governing the technology. "The principles are for application around the world, as part of a drive to try and harmonize international regulation," Hynes said.

That is all very well, but as Gary Marchant, of Arizona State University, also a member of the committee, noted, "There is a huge problem of international enforcement." Ways of trying to ensure compliance considered in the report include journals only publishing research that complies with international norms and withholding research grants if the rules are not upheld.

The committee did not consider patenting in its survey of gene editing. However, Marchant suggested refusing licenses could be another mechanism in attempts to ensure appropriate use of the technology worldwide. That "may have more currency at an international level," he said.

See the rest here:
There's a fine red line between cures, enhancements using gene editing tech - BioWorld Online

Rio Rancho High School senior baseball standout Garrett Gouldsmith knows the meaning of sacrifice, not only as it pertains to baseball but also in life.

You see, Gouldsmith, 17, once donated bone marrow to his younger sister Gabby (now 13 and a student at Lincoln Middle School); that sacrifice saved her life. The family was living in Reno at the time; Buddy and Heather Gouldsmith moved their family to Rio Rancho in August 2015.

Five years ago my sister was diagnosed with severe aplastic anemia, he said, sounding like Doogie Howser.

Aplastic anemia occurs when bone marrow does not produce enough stem cells due to damage. These stem cells develop into the different types of blood cells, including red blood cells, which deliver oxygen to other cells of the body. Mild to moderate cases may not need treatment, but severe cases may result in death and require emergency care.

It is genetic, Gouldsmith said. Its a one-in-five million chance of developing this. It is a super-rare disorder.

Originally, there wasnt really a plan for her treatment, other than a sibling bone marrow (transplant), and they had to be a perfect seven-out-of-seven HLA (human leukocyte antigen) match, which basically means that they have to have the same seven blood-marker identifiers.

So me and my brother (Gunner) got tested. Gunner was a six-out-of-seven match; I was a seven-out-of-seven match, which was a major blessing, a dream come true, he said. So about a week later, after they went through some testing and I finished up playing in Little League districts, I went and donated bone marrow on July 15, 2011. And that was after she went through her rigorous chemo treatment.

After she received the bone marrow, her numbers in terms of T cells, her white blood cells, her red-blood cells all the numbers started to go up, he explained. Everythings been going in a positive way and this past December, right before Christmas, we got the greatest Christmas present of all time: She was officially considered out of remission and cured.

If the baseball future pales, he said, theres no plan to enter the medical field: It was five years of talking about this stuff every single day, he said, that led to his expertise on the subject.

What about that bone marrow donation?

In terms of my baseball career, Id take my sisters life over my baseball career every single day, Gouldsmith said. At the time, it was just, Hey, lets do what we have to do to so that my sister can get healthy again and we can get back to as normal a life as possible.

I was sedated for about two-and-a-half, three hours, he recalled. The treatment itself is three little tiny holes in your lower back, and then they fan with a needle about 150 times in each hole and extract bone marrow.

Being sedated at the time, the bone-marrow removal was painless, but, he said, I felt it later, for about the next week.

After they do that, they put it in a little bag, send it off in an ambulance to a lab so they can do what they need to do in terms of filtering it, he said. And then its put into an IV bag and it just goes into her like an IV.

At the time, back in Nevada, She lost all her hair; she had to wear a mask for the better part of two years, wherever she went, he said. Inside, in terms of our house lifestyle, she could only eat certain foods, if it was packaged, frozen.

The survival rate for patients with aplastic anemia under age 20 who have a bone marrow or stem cell transplant is 80 percent, according to St. Jude Childrens Research Hospital.

With that nightmare and its happy ending behind him, Gouldsmith said hed love to get back to the Class 6A championship game at Isotopes Park in what would be his final game as a Ram.

Thats where he played his last game with the Rams, stranded on second base in the bottom of the ninth, the potential tying run when Wille Baez looked at a third strike to end the extra-inning 2-1 loss to Carlsbad.

The 2016 season was Gouldsmiths first in New Mexico, but it wont be his last: He was an early signer to play at the University of New Mexico, where his father is an assistant coach. Last years District 1-6A Player of the Year, he said he chose UNM over Nevada (back in his hometown of Reno), UCLA and Arizona because of the Lobos success on the field and their success in developing players and moving them on to the next level.

Thats right: Gouldsmith would love to play Major League Baseball, like his shortstop idol, Derek Jeter.

We play the same position, Gouldsmith said. I love the way he did things, both on and off the field. He kept to himself, stayed out of trouble off the field and was a superstar on the field.

Obviously, we dont have the same body type (The Lobos list their recruit at 5-foot-9, 160 lbs.), he said, then likening himself to Houston Astros shortstop Jose Altuve (5-6, 165; the shortest player in the majors).

Murphy and Gouldsmith are thankful the season opener is still a few weeks away (Feb. 23 in El Paso; the starting shortstop was injured at of all places a Lobo baseball camp.

Playing in a scrimmage, I just dove for a backhand, he explained. I didnt think anything of it at the time. I originally came out of the game because I jammed my thumb diving for that ball.

I just kind of sat out, then went home and tried to grab a water out of the fridge and couldnt lift my left arm, he said. Doctor said that nothing surgical needs to go on so I just have to give it some time before we head off to El Paso for our first tournament of the year.

Gouldsmith was expected to get the doctors clearance to practice and play this past Thursday.

Gouldsmith knows theres a lot of hard work ahead of him, to make that MLB dream come true.

Murphy terms him the best five-tool player hes ever had, although Gouldsmith downplays the power tool of that description.

He batted .562 with three home runs, 12 doubles and five triples, with just three errors in 108 total chances.

My goal for this season, obviously, is to win a state championship, but over and above that is to raise money to give back to the community for all the help they gave us and our family during that time.

My plan for this season, to kind of give back to the community in ways the community gave to us when we were going through this, is to have people donate money in terms of a pledge donation or just a flat donation for every base I steal to the Jessie Rees Foundation (negu.org).

(Jessie Rees was a beautiful, athletic, smart and compassionate 12-year-old girl who bravely fought two brain tumors for 10 months and two days. Her fight started March 3, 2011, and ended Jan. 5, 2012, when she lost her battle. During her courageous fight, Jessie decided to focus on helping other kids fighting cancer that couldnt leave the hospital.)

Their phase is Never Ever Give Up, he said. (Ill) donate all the proceeds I receive to them.

Our plan is to, obviously, respect the game first (He sounds like Jeter here.) and steal when necessary and helpful to the team first, he said. Our coaching staff and players have been really supportive of what Im trying to do, and Ive been very grateful to them.

Garrett almost lost his sister, Murphy said. It becomes real personal for the whole team, because now it hits home this is one of us.

It makes it that much more important for us to help him out, he said. We tell these kids all the time that playing baseball is a very small part of this program We really pride ourselves in the community work we do work with the policemen, work with the fire department, doing things for people in the community and this is just another way for us to prove that.

Baseballs still a game. There are so many things in life that are so much more important than baseball, like this, Murphy said. This is what we want our kids to learn about. You can use baseball not only to have fun and play the game, maybe get a college scholarship out of it, but you can also use it for a lot of other good things, like this cause.

To contribute to Gouldsmiths Steal 4 Joy fund, make a one-time pledge or pledge-per-stole-base via head coach Ron Murphy at ron.murphy@rrps.net.

Gouldsmith swiped 31 bases last season, but Murphy wont be giving him the green light every time No.8 is on base.

What you have to understand is were not out there, just trying to steal mucho bases, its still underneath my guidelines, me coaching third and the steal sign and the green light.

Excerpt from:
Ballplayer's sacrifice saves his little sister - Rio Rancho Observer

By MATTHEW NASH

Peninsula Daily News

SEQUIM Scientists at the Pacific Northwest National Laboratorys Marine Sciences Laboratory are turning algae inside out to find the best biofuel possible.

Dr. Michael Huesemann, a lead researcher, said algae is a promising clean energy but expensive to harness effectively.

The price of biofuel is largely tied to growth rates, he said. Our method could help developers find the most productive algae strains more quickly and efficiently.

Huesemann, who has worked with algae for 16 years, started on the $6 million, three-year algae DISCOVR project (Development of Integrated Screening, Cultivar Optimization and Validation Research) in October 2016 with a small team of senior and junior scientists in Sequim, reported the Peninsula Daily News.

What were really interested in are carbon-neutral biofuels that can be grown in the United States, he said.

We havent found any algae that grows fast enough or provides enough biofuel, so were trying to find microalgae that grows fast to make biofuels.

Huesemann said there are millions of strains in nature to sort through, so rather than a shotgun approach and randomly screening they are doing an organized, rational screening.

Finding the best algae is broken into a process of five tiers among labs in Sequim, three other Department of Energy labs Los Alamos National Laboratory, National Renewable Energy Laboratory, Sandia National Laboratories and Arizona State Universitys Arizona Center for Algae Technology and Innovation.

Previously, researchers tried to evaluate algae in test tubes but find lab results dont always mirror outdoor ponds, laboratory officials said.

In Tier I, scientists in Sequim and New Mexico test up to 30 different algae strains to see how weather-tolerant they are, and the top third will go to Tier II, Huesemann said.

In Tier II, Sequim houses a unique climate-simulating system called LEAPS (Laboratory Environmental Algae Pond Simulator) that simulates climates and seasons around the world inside glass cylinder photobioreactors.

Two other labs will evaluate the algae to see if there is any value in its compounds that could be used for products like food dye phycocyanin, which could make algae biofuel production more cost-effective, Huesemann said.

Scientists also will research how resilient certain algae strains are to predators, like protozoans, and other competing algae.

Huesemann anticipates Tier I and II being conducted this year, he said.

For Tier III, researchers in New Mexico will further test top-performing algae strains, Huesemann said, which includes forcing cells to grow faster or generate more oils, using state-of-the-art laboratory techniques such as directed evolution and fluorescence-assisted cell sorting.

Afterward, strains will travel to outdoor ponds in Arizona for study as part of Tier IV to compare biomass output with earlier steps. Lastly, scientists will study the algae strains that performed the best in different lighting and temperature conditions for Tier V.

Data will go into the Pacific Northwest National Laboratories Biomass Assessment Tool to help researchers generate maps that illustrate the expected biomass productivity of each algae species grown in outdoor ponds all over the U.S. in different seasons on any day of the year, Huesemann said.

Once the study is complete, research will be made public for companies and other researchers.

Laboratory officials said work that could stem from this project includes converting harvested algae into biofuels, examining operational changes such as crop rotation to further increase biomass growth and assessing the technical feasibility and economic costs of making biofuel from algae selected through this process.

See the original post:
Sequim lab looks to find the best biofuel in algae - The Daily Herald

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Small sheets of healthy skin are being grown from scratch at a Stanford University lab, proof that gene therapy can help heal a rare disease that causes great human suffering.

The precious skin represents growing hope for patients who suffer from the incurable blistering disease epidermolysis bullosa and acceleration of the once-beleaguered field of gene therapy, which strives to cure disease by inserting missing genes into sick cells.

It is pink and healthy. Its tougher. It doesnt blister, said patient and research volunteer Monique Roeder, 33, of Cedar City, Utah, who has received grafts of corrected skin cells, each about the size of an iPhone 5, to cover wounds on her arms.

More than 10,000 human diseases are caused by a single gene defect, and epidermolysis bullosa is among the most devastating. Patients lack a critical protein that binds the layers of skin together. Without this protein, the skin tears apart, causing severe pain, infection, disfigurement and in many cases, early death from an aggressive form of skin cancer.

The corrected skin is part of a pipeline of potential gene therapies at Stanfords new Center for Definitive and Curative Medicine, announced last week.

The center, a new joint initiative of Stanford Healthcare, Stanford Childrens Health, and the Stanford School of Medicine, is designed to accelerate cellular therapies at the universitys state-of-the-art manufacturing facility on Palo Altos California Avenue. Simultaneously, itisaiming to bring cures to patients faster than before and boost the financial value of Stanfords discoveries before theyre licensed out to biotech companies.

With trials such as these, we are entering a new era in medicine, said Dr. Lloyd B. Minor, dean of the Stanford University School of Medicine.

Gene therapy was dealt a major setback in 1999 when Jesse Gelsinger, an Arizona teenager with a genetic liver disease, had a fatal reaction to the virus that scientists had used to insert a corrective gene.

But current trials are safer, more precise and build on better basic understanding. Stanford is also using gene therapy to target other diseases, such as sickle cell anemia and beta thalassemia,a blood disorder that reduces the production of hemoglobin.

There are several diseases that are miserable and worthy of gene therapy approaches, said associate professor of dermatology Dr. Jean Tang, who co-led the trial with Dr. Peter Marinkovich. But epidermolysis bullosa, she said, is one of the worst of the worst.

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It took nearly 20 years for Stanford researchers to bring this gene therapy to Roeder and her fellow patients.

It is very satisfying to be able to finally give patients something that can help them, said Marinkovich.In some cases, wounds that had not healed for five years were successfully healed with the gene therapy.

Before, he noted, there was only limited amounts of what you can do for them. We can treat their wounds and give them sophisticated Band-Aids. But after you give them all that stuff, you still see the skin falling apart, Marinkovich said. This makes you feel like youre making a difference in the world.

Roeder seemed healthy at birth. But when her family celebrated her arrival by imprinting her tiny feet on a keepsake birth certificate, she blistered. They encouraged her to lead a normal childhood, riding bicycles and gentle horses. Shes happily married. But shes grown cautious, focusing on photography, writing a blog and enjoying her pets.

Scarring has caused her hands and feet digits to become mittened or webbed. Due to pain and risk of injury, she uses a wheelchair rather than walking long distances.

Every movement has to be planned out in my head so I dont upset my skin somehow, she said. Wound care can take three to six hours a day.

She heard about the Stanford research shortly after losing her best friend, who also had epidermolysis bullosa, to skin cancer, a common consequence of the disease. Roeder thought: Why dont you try? She didnt get the chance.

The team of Stanford experts harvested a small sample of skin cells, about the size of a pencil eraser, from her back. They put her cells in warm broth in a petri dish, where they thrived.

To this broth they added a special virus, carrying the missing gene. Once infected, the cells began producing normal collagen.

They coaxed these genetically corrected cells to form sheets of skin. The sheets were then surgically grafted onto a patients chronic or new wounds in six locations. The team reported their initial results in Novembers Journal of the American Medical Association.

Historically, medical treatment has had limited options: excising a sick organ or giving medicine, said Dr. Anthony E. Oro of Stanfords Institute for Stem Cell Biology and Regenerative Medicine. When those two arent possible, theres only symptom relief.

But the deciphering of the human genome, and new tools in gene repair, have changed the therapeutic landscape.

Now that we know the genetic basis of disease, we can use the confluence of stem cell biology, genome editing and tissue engineering to develop therapies, Oro said.

Its not practical to wrap the entire body of a patient with epidermolysis bullosa in vast sheets of new skin, like a mummy, Oro said.

But now that the team has proved that gene therapy works, they can try related approaches, such as using gene-editing tools directly on the patients skin, or applying corrected cells like a spray-on tan.

A cure doesnt take one step, said Tang. It takes many steps towards disease modification, and this is the first big one. Were always looking for something better.

Here is the original post:
Stanford team is growing healthy skin for ill patients - The Mercury News

BUCKEYE, AZ - As the saying goes: "blood is thicker than water." But when it comes to bone marrow, it is truer than ever especially because family is usually the only people to turn to for a match.

But, one Buckeye family is finding that the phrase could not be more perfectfor them because a brother has been serving as the lifeline for his sibling over the last few years.

Gloria Mesquias calls her 11-year-old son, Shaun, "the warrior."

"He takes every jab he gets and just rolls through," said Mesquias.

Shaun's 13-year-old brother Malik is called "the hero."

"They are actually like night and day, "Mesquias said. "They're brothers."

The three of them, and other supportive family members, have spent months at Phoenix Children's Hospital. Shaun has a condition where his body isn't producing new blood cells.

"He was diagnosed with severe aplastic anemia," Mesquias said.

That happened when he was just about 1 year old. Ever since then, he's been in and out of the hospital.

But, Malikhas served as a bone marrow and stem cell donor to his b brother not once, not twice but, three different times.

And the fight for this little warrior, is not over yet. If his treatment goes well over the next few days, he will have a fourth surgery on Wednesday; another stem cell transplant.

Mesquias doing this all as a single mother. She also has a 5-year-old daughter, who has not seen Shaun since before Christmas.

"She understand;she gets it," Mesquias explained. "She knows brother is sick and she knows mom is here with Shaun."

But, while she tries to keep it together, all of the stress and days away from home are weighing on Mesquias. But, it's something she will never let her family see.

"At night time, I can go in the restroom and cry my eyes out or ball my face out in the pillow," Mesquias said. "But, I just don't do it in front of him."

So, the boys' Buckeye teacher, Carrie Brown, has also taken action to try and do something special for the family.

"She would never ask for help," Brown said. "She's not that kind of person. So, I just thought that this was one thing that I could do to relieve some of the worry that she has and to give her a little bit of comfort."

Brown started a GoFundMe page to try and help the family who has given so much to each other.

And Mesquias said she is making sure all of them get out of that hospital together.

"He has his moments too where he says he wants to go home," Mesquias explained. "And... I'm like, 'I'm not going home until you're going home. So, we're good."

The rest is here:
Buckeye boy donates bone marrow to sick brother - ABC15 Arizona

At the Arizona Center for Advanced Medicine, we can help those suffering from cancer with holistic, comprehensive treatment that addresses every angle of the disease.* At our Scottsdale integrative medical center, we can offer you a full-range of medical support through this difficult time. Learn more about the different approaches we take to helping patients recover from cancer, or contact us directly.

A targeted approach using low-dose chemo and complementary therapies to both defeat cancer and rebuild the immune system.*

The conventional treatment for cancer is the familiar trio of chemotherapy, radiation, and surgery. The subsequent devastation to the immune system and the organs - especially the liver and heart - is significant. The reduced quality of life, including losing one's hair, vomiting, diarrhea, mouth ulcers, low white count, susceptibility to infection, damage to the immune system, etc. is also pretty hard to take.

In conventional treatment, chemotherapy drugs must be administered in doses high enough to kill a large number of cancer cells without killing the body's immune system and intestinal tract. It's a balancing act. Patients are given as much chemotherapy as their body can tolerate. It is like killing flies with a cannonball instead of fly swatter. You get rid of some flies, yes, but you have a lot of collateral damage. Good cells die along with the bad. Over time (and sometimes a fairly short period of time, days rather than weeks), this massive bombardment can lead to extremely low blood counts, organ failure, and death. Because an already poorly functioning immune system is subjected to radiation and toxic drugs, it is difficult to deliver a "cure."[1]

A time-tested, modified form of chemotherapy has been used successfully and safely around the world for more than 70 years. It is called IPTLDSM, or Insulin Potentiation Therapy Low Dose.

IPTLD uses between 10 and 25% of the dosage of a conventional chemotherapy regime. It provides a safer, much gentler alternative to conventional chemotherapy, without the harsh side effects.* When combined with complementary therapies to nurture the body, it is also more effective. It is a smart way to approach cancer based on what makes cancer cells vulnerable.

There is a key difference between cancer cells and healthy cells: cancer cells run exclusively on sugar and glutamine, the amino acid found in high concentration in animal proteins. Cancer cells have a ravenous need to consume the glucose (sugar) found in the blood stream. Glucose is their unique source of energy, and because of the relatively inefficient way cancer cells burn this fuel, they use up a great deal of it. This is why cancer patients lose so much weight. Because cancer cells require so much glucose, they virtually steal it away from the body's normal cells, thus starving them.

To help sugar get inside the cancer cells, they are equipped with 10-16 times as many insulin receptors as healthy cells.[2,3] Insulin manages the delivery of glucose across cell membranes into the cells. Put another way, insulin escorts glucose through the cell membrane, into the interior where the glucose provides energy to keep the cell alive.

If you have had a PET scan, you have seen this connection between sugar and cancer cells at work. A PET scan is performed by injecting a radioactive agent attached to a glucose molecule into a vein. The cancer cells, always ravenous for sugar, take up the glucose much faster than healthy cells. The radioactive agent gets into the cell along with the glucose. Bingo - the scan produces a three-dimensional picture of a cancerous mass.

Call our Scottsdale integrative medical center at (480) 418-0220 to learn more about how we can help.

Now, what would happen if, in addition to glucose, you add a little bit of chemotherapy to the mixture? Bingo - the chemotherapy drugs are dragged in to the cancer cells along with that glucose that they so crave. The healthy cells are not bombarded. This is why patients undergoing IPT do not experience the severe side effects of conventional therapy. Generally, IPT patients do not go bald, nor do they experience severe nausea or organ damage.

The word "potentiate" means to make stronger or more effective. In this case, it means that insulin makes the chemotherapy more effective. A 1981 study conducted at George Washington University showed that when the chemotherapy drug, methotrexate, is combined with insulin, the drug's cell-killing effect increased by a factor of 10,000.[4] Because insulin enhances the effectiveness of the drugs, IPT uses only 10% to 25% of standard dose drugs. There is no need to overwhelm a patient with large quantities of drugs in the hope that the drugs will kill the cancer before they kill the patient's immune system.

There is a second way that insulin helps us defeat cancer. Insulin stimulates cells to grow, which they do by dividing. Cancer cells are most vulnerable to many chemotherapeutic agents when they are dividing.[5] With IPT, we use insulin's stimulating properties to catch more cancer cells in the process of dividing, so more of the drugs are absorbed than if division had not been encouraged.

A third way insulin helps is with detoxification. Insulin increases "cellular permeability." Glucose goes in easier, and the low dose chemo goes in easier. The door swings both ways - toxins and debris from dying tumor cells also pass out much easier. Insulin facilitates the detoxification so necessary with cancer.

There is universal agreement that cancer is a failure of the immune system. We feel strongly that "killing the cancer" is only one part of the job. Re-training and strengthening the immune system is another part of our job. Our patients continue to thrive and often end up with a healthier immune system than when they started treatment.*

We use complementary therapies to nourish the body and protect the organs, to create a more robust immune system and incidentally to create a hostile inner terrain for cancer.*

As a tumor grows, the body may acclimate to the presence of abnormal cell growth, fooling the brain into accepting the cancer as a normal presence in the system. After the tumor is removed, the body may still respond as if it were still present, much like an amputee's "phantom limb" syndrome.

One school of thought maintains that the brain sends messages that support re-growth of tumors. Another school of thought teaches that each organ system reflects particular emotions, both positive and negative. When illness strikes, generally the cause originates in the mental or emotional sphere, and manifests in that part of the body which is most vulnerable.

So, for instance, if we have been sexually abused as children, we may very likely develop cancer of the female organs. If we see ourselves as powerless, we may well develop cancer of the pancreas. If we are full of fear, bladder or kidney cancer is often the end result.

It's not that we intend to become ill, not at all. But our bodies will hold the messages of those experiences that we are too small or too powerless to deal with. We hold those messages in the form of neurotransmitters, second messengers, inflammatory markers, muscle tension, and habitual responses to situations. If our parents were alcoholic, we tend to marry alcoholics. If our parents beat us, or put us down all the time, we tend to marry people who also beat us and denigrate us.

That does not mean that we cannot change our attitude. But first, we have to see our attitude for what it is.

Infrared saunas offer another approach to defeat cancer, through the use of elevated body temperature. When fighting pathogens, the body sometimes creates a fever to raise the internal temperature to kill unwanted cells. We use the same principle with infrared saunas. Also, saunas are a great way to detoxify. As a tumor shrinks, it sheds a large quantity of debris. Saunas assist the body's efforts to move out the toxins through the sweat.*

The Photon Genius is a form of infrared sauna which not only uses heat - like a regular sauna - but also coherent light frequencies, to restore a sense of order to the cells of the body, as the light is carried around by our own red blood cells.

At our Scottsdale alternative cancer treatment center, we use nutritional therapy with all our patients. The cornerstone is a low glycemic diet - very low sugar loads, no refined foods, nothing in a box, nothing with chemicals or colorings in it. And you would be surprised how much of the supermarket is devoted to things with chemicals and colorings. Take a look at the snack and cracker aisle some time. We also use anti-angiogenic therapy - largely through diet, consumption of those foods which heal the blood vessel walls and decrease their responsiveness to the siren calls of tiny tumors for more blood.[10,11] All our patients are encouraged (and taught) to do vegetable juicing every day. We help them learn how to cook, if necessary.* After all, how many people have been on a gluten-free diet for 20 years? Certainly not most of those who come to our office.

An important part of the therapy involves fasting, to promote starvation responses in the tumor cells. Since normal cells have a protective mechanism that shuts down their metabolism when fuel is scarce, they are protected when food is in short supply.[12] Fasting the day before chemotherapy is highly recommended, and fasting for at least 8-10 hours before IPTLDSM therapy is required at the Arizona Center for Advanced Medicine.

After administering IPT, along with vitamins, herbs, immune enhancers, and chelation, repeat testing 4-6 weeks later will often show that the cancer has regressed, or even disappeared.

The culprit behind perhaps half the cancer deaths is a wasting syndrome called cachexia (pronounced "ka-kek-see-ah"). Patients lose weight and literally starve to death.

Because cancer cells need even more energy than regular cells, the cancer cells gobble up the incoming nutrition first. Your healthy cells get what is left over which can mean the rest of your cells starve when conventional treatment leaves you too nauseated to eat. The tumor stays strong, but the patient wastes away.

The hypoglycemic pulse that occurs with the administration of insulin actually helps the body assimilate the nutrition in food - vitamins, minerals, and enzymes. Because IPT is a gentler approach, patients do not get the severe bouts of nausea so common with conventional chemotherapy due to destruction of the rapidly dividing and always renewed cells lining the intestinal tract.

Which chemo drugs shall we use? Which complementary therapies are right for you?

Unlike conventional chemotherapy treatment, IPTLD is not a one-size-fits-all approach. You are unique, and your response to various drugs and complementary therapies is not necessarily the same as the next person's.

Think back to a time when you had a bladder infection. The lab tested your urine sample against different antibiotics to find out which ones were most effective at killing the bacteria. We use the same concept when choosing therapies for chemotherapy.

We take a sample of your blood (and a fresh tissue sample, if available) and test it against the chemo drugs and the various complementary therapies to find out what will be most effective for you. We also look at the genetic makeup of your individual tumor. When we custom tailor your therapy, you have a better result.*

We use a laboratory which does the actual cell cultures in Greece, although it has branches all over the world. The test is not inexpensive, and insurance usually does not pay for this test. But we strongly feel it is the best money you will ever spend. We include this test in our charges for the initial 8 weeks of treatment.

The RESEARCH GENETIC CANCER CENTRE LTD is headed by Dr. Ioannis Papasotiriou, an oncologist. He has developed a way of isolating circulating tumor cells from peripheral blood, growing them in cell culture, and testing them for chemosensitivity, as well as sensitivity to alternative/biological/botanical agents.

Only a few populations of tumor cells actually develop the ability to invade other tissues and create metastatic spread of tumor. These cells are known as circulating tumor cells (CTCs) and many of them are cancer stem cells. The RGCC test isolates and measures these cells, then grows them in such a way that they do not mutate during the growth process. The cells can then be tested against various chemotherapeutic agents, as well as many alternative/botanical/nutrient remedies.

Also, metastatic cancer cells can vary genetically from the primary tumor. At least two studies with breast cancer patients have demonstrated that CTC can be HER2 positive while the primary breast tumor can be HER2 negative.[14,15] Another study with prostate cancer demonstrated the same phenomenon. [16]

A landmark study published in the New England Journal of Medicine in 2007 compared women with lymph node-positive breast cancer who received the standard trio of chemotherapy drugs - Adriamycin, Cytoxan, and Taxol (called ACT) to women who did not receive any chemotherapy. Their HER2 status was also determined - the genetic characteristic of the cancer. Researchers discovered that women who were HER2 negative and estrogen receptor positive did not benefit at all from taking Taxol.[17] Because approximately two thirds of women with breast cancer fall into this category, the ramifications of this study are immense. So much for the ineffectiveness of the one-size-fits-all approach to cancer.

A study published in the Journal of the National Cancer Institute in 2008 measured the effectiveness of an anthracycline-based chemotherapy regimen in 5,354 women with early-stage breast cancer. Anthracyclines are a class of chemotherapy drugs of which Adriamycin is a key member. Scientists determined that women with early-stage breast cancer who were HER2 negative derived absolutely no benefit from taking Adriamycin or other anthracycline drugs.[18] Given that approximately 80% of breast cancers are HER2 negative, then only 1 out of 5 women with breast cancer can benefit from these drugs that have considerable toxicity associated with their use. In another study, 7% of patients treated with Adriamycin developed congestive heart failure.[19] They apparently did not have Poly-MVA available to them.

With standard chemotherapy, treatments are often spaced several weeks apart, to allow the body to recover from the harsh effects of the treatment. Since the standard dose chemotherapy attacks all rapidly dividing cells, almost every patient experiences hair loss, and frequently they develop diarrhea and intestinal tract ulcerations as well. Treatment is often limited by the number and severity of the "side effects" which the patient experiences. In addition, the length of time between treatments allows the tumor cells which were not killed initially to continue growing. Sometimes the cells not killed become resistant to the chemotherapeutic agent which was used, and by spacing the treatments so far apart (in order to protect the life of the patient) the resistant population of cells is allowed to take over.

IPTLDSM is a more enlightened paradigm which tailors treatment towards the individual uniqueness of your body and your cancer. With IPT therapy, we generally start with low-dose chemotherapy agents twice a week for the couple of weeks. The frequency may then be reduced to once a week, and eventually to once every 2-3 months, until there is no further sign of cancer cells in the blood, and the tumor is no longer visible by conventional means. Chemotherapy agents are interspersed with complementary therapies. So on Monday, for example, you may receive chemotherapy agents and on Wednesday, you may receive intravenous vitamin C.

We encourage you to request an orientation with our Scottsdale alternative cancer treatment center so you can make an educated decision. There is no charge for this. Come meet the doctors and staff, and tour the clinic. Meet other patients. The course of action you choose is a significant commitment, and one that will impact those around you. Feel free to bring family to the orientation. Ask every question that is on your mind.

For your first appointment (after the orientation visit), plan to spend two hours with us. Bring all your medical records and test results. We will ask you to fill out a history form ahead of time so you can do that at home where you have access to information about prior vaccinations, surgeries, mercury fillings and root canals, major emotional traumas you have experienced in life, etc.

We have an integrative cancer therapy program that provides an approach for the short and the long term - the treatment is tough enough to defeat cancer in the short term, yet leave your body nourished and empowered to ward off cancer on its own in the long term so the cancer does not return.*

"Nothing in life is to be feared. It is only to be understood." - Marie Curie, awarded Nobel Prizes in physics and chemistry

*Disclaimer: There is no guarantee of successes for any given medical treatment. Each individual is unique and may respond differently to our medical services, meaning results may vary for each person.

[1] Morgan G, Ward R et al. The contribution of cytotoxic chemotherapy to 5-year survival in adult malignancies. Clin Oncol (R Coll Radiol). 2004 Dec;16(8):549-60.

[2] Milazzo G, Giorgina F, Belfiore A et al. Insulin Receptor Expression and Function in Human Breast Cancer Cell Lines. CANCER RESEARCH 52, 3924-3930, July 15, 1992

[3] Belfiore A, Malaguarnera R. Insulin receptor and cancer. Endocr Relat Cancer. 2011 Jul 4;18(4):R125-47. doi: 10.1530/ERC-11-0074.

[4] Alabaster, A; Vonderhaar, B; Shafie S. Metabolic modification by insulin enhances methotrexate cytotoxicity in MCF-7 human breast cancer cells. Eur J Cancer Clin Oncol. 17:1223-1228

[5] Scavo LM, Karas et al. Insulin-Like Growth Factor-I Stimulates Both Cell Growth and Lipogenesis during Differentiation of Human Mesenchymal Stem Cells into Adipocytes. J Clin Endocrin Metab 89;7:3542-3552.

[6] Litsey T. Acupuncture vs. Cancer: Re-Engaging the Body's Immune System . AcupunctureToday. October, 2003, Vol. 04, Issue 10

[7] http://mosao2.org/Article%20-%20Medicine/cancer_Otto_Warburg_00.pdf Downloaded 02-24-13.

[8] Chen Q, Espey MG, Krishna MC, Mitchell JB, Corpe CP, Buettner GR, Shacter E, Levine M. Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. Proc Natl Acad SciU S A. 2005 Sep 20; 102(38):13604-9.

[9] Sudheesh NP, Ajith TA, Janardhanan KK, Krishnan CV. Effect of POLY-MVA, a palladium alpha-lipoic acid complex formulation against declined mitochondrial antioxidant status in the myocardium of aged rats. Food Chem Toxicol. 2010 Jul;48(7):1858-62. doi: 10.1016/j.fct.2010.04.022.

[10] Li WW, Li VW, Hutnik M, Chiou AS. Tumor angiogenesis as a target for dietary cancer prevention. J Oncol. 2012;2012:879623. doi: 10.1155/2012/879623.

[11] Arulselvan P, Wen CC, Lan CW, Chen YH, Wei WC, Yang NS. Dietary administration of scallion extract effectively inhibits colorectal tumor growth: cellular and molecular mechanisms in mice. PLoS One. 2012;7(9):e44658.

[12] Lee C, Longo VD. Fasting vs dietary restriction in cellular protection and cancer treatment: from model organisms to patients. Oncogene. 2011 Jul 28;30(30):3305-16. doi: 10.1038/onc.2011.91.

[13 Apostolou P, Toloudi M, Chatziioannou M, Ioannou E, Papasotiriou I. Cancer stem cells stemness transcription factors expression correlates with breast cancer disease stage. Curr Stem Cell Res Ther. 2012 Nov;7(6):415-9.

[14] S Meng, D Tripathy, et al; HER-2 gene amplification can be acquired as breast cancer progresses. Proc Natl Acad Sci U S A. June 22, 2004;101(25):9393-8.

[15] P. Wlfing, J Borchard, et al; HER2-positive circulating tumor cells indicate poor clinical outcome in stage I to III breast cancer patients. Clin Cancer Res. March 15, 2006;12(6):1715-20.

[16] CA Olsson, GM De Vries, et al; The use of RT-PCR for prostate-specific antigen assay to predict potential surgical failures before radical prostatectomy: molecular staging of prostate cancer. Br J Urol. March 7, 1996;7(3):411-7.

[17] DF Hayes, AD Thor, et al; Cancer and Leukemia Group B (CALGB) Investigators. HER2 and response to paclitaxel in node-positive breast cance r. New England Journal of Medicine, October 11, 2007;357(15):1496-506.

[18] A Gennari, MP Sormani ,et al; HER2 status and efficacy of adjuvant anthracyclines in early breast cancer: a pooled analysis of randomized trials. J Natl Cancer Inst. January 2, 2008; 100(1):14-20.

[19] SM Swain, FS Whaley, MS Ewer; Congestive heart failure in patients treated with doxorubicin: a retrospective analysis of three trials. Cancer. June 1, 2003; 97(11):2869-79.

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Scotssdale Cancer Treatment Center | AZ Integrative Medicine

Studio City, CA (PRWEB) December 4, 2007 (years ahead of the curve on this)

Politicians will ask us to allocate billions for stem cell research without having a clue as to what is possible, probable or even feasible. As example, we were told treatments for heart disease; spinal injury, Parkinson's, blindness, MS and diabetes could only come from embryonic stem cells. The truth is, not one treatment for these diseases has come from an embryonic stem cell. Instead, successful treatments for each of the ailmnents are coming from (non-embryonic) adult stem cells. http://www.abigon.com Why has the public not been made aware of these important breakthroughs? Isn't it time we receive accurate, factual information as we are asked to help guide what might arguably be the most important medical advancement in medical history?

Finally, last week, revered embryonic scientific stem cell leader Ian Wilmut (cloner of Dolly the sheep) made an announcement in support of adult stem cells over embryonic that stunned the entire stem cell research community. The professor did not base his conclusions on religious or moral preference but on solid scientific principal. As a matter of fact, Professor Wilmut has never believed embryonic research to be unethical, he simply concluded, there is a better way to accomplish the objective without destroying the embryo. http://www.abigon.com

Earlier this year, neurologist Dr. Carlos Lima, addressing the House of Lords in England reminded all in attendance that even though government funding for embryonic research had been limited in both England and the US, research has been going on unfettered for more than 10 years in the rest of the world including China, Russia, Korea, India and Germany. Embryonic research has not produced a single successful documented treatment. http://www.abigon.com

Perhaps nowhere has the case for adult cells been previously made more succinctly than in author/researcher Christian Wilde's new breakthrough book, Miracle Stem Cell Heart Repair. As the author explains; "If you take the moral, political and ethical concerns off the table, the scientific issues alone confronting ESC research (according to many scientists) are in themselves, daunting. Before an ESC can be safely injected into a human being it must be proven safe in an animal study. In cases to date, animal subjects have experienced dangerous tumor growth and rejection by the body." He believes the public deserves balanced non-selective and un-biased reporting on both forms of stem cell research. http://www.abigon.com

Currently Heart Failure is claiming the lives of 22 million victims world wide with a one to seven year life expectancy and 50% not making the five-year mark. There is good news a-coming, Miracle Stem Cell Heart Repair documents "no option" heart patient's stories who have successfully transitioned within the FDA trials from near death to recovery following a one time, minimally invasive procedure using their own thigh muscle stem cells. Why, asks Wilde, should it be headline world news that someday an embryonic stem cell might possibly heal a mouse heart but nowhere is there a headline that proclaims hundreds of actual living breathing people, (not mice) have already had their damaged hearts repaired with adult stem cells? Is this a case of selective reporting? http://www.abigon.com

Several of the patients in the study treated at Columbia and Arizona Heart Institute,whose stories are recorded in the book had survived as many as five previous heart attacks. As one patient's own physician told him, frankly I don't know how you are even alive, 75% of your heart is not functioning. Following the one-time autologous treatment with the patient's own stem cells at Arizona Heart, performed by Nabil Dib, M.D., Chief of Cardiovascular Research (currently Director of Cardiovascular Stem Cell Therapy at UCSD) the patient was walking one mile and then two miles a day in a literal matter of weeks.

A simultaneous study done at Columbia, Minneapolis Heart, Cleveland Clinic, Mayo Clinic and St. Joseph's has confirmed similar favorable results and the MARVEL (follow-up) study has now been approved to enroll up to 390 more patients as treatment in the two studies moves closer to approval. The first patient to receive his own stem cells in the US almost four years ago continues to do well. This first procedure was performed by M.D., Warren Sherman, Director of Cell-Based Endovascular Therapies at Columbia and Chief Investigational Officer of the expanded FDA study.

Adult cells are being used currently to treat Parkinson's, blindness, traumatic brain injury, MS, several cancers, type I&II diabetes and more than 72 diseases. Four hundred fifty blind patients in India and eight patients per month in Cincinnati are being cured of corneal blindness. A type I diabetic patient in London has been insulin free for three years following a one-time injection of bone marrow stem cells to the pancreas and type II diabetes are similarly successfully being treated in Argentina and Brazil using adult stem cells. http://www.abigon.com

Spinal injury: One hundred five quadriplegic and paraplegic patients are beginning to walk (some with braces) following a single procedure in which stem cells from their own nasal olfactory cavity were harvested and injected into the areas of spinal lesion. One-half of Dr. Lima's patients are from the US. http://www.abigon.com

Miracle Stem Cell Heart Repair contributors are eight of the world's leading stem cell scientists serving as directors of cardiovascular cell therapy from the universities of Columbia, UCSD, Pittsburgh, Minnesota, Tufts, Johns Hopkins, Cedars Sinai Medical Center, Arizona Heart and Minnesota Heart Institutes and directing the ongoing FDA trials.

Christian Wilde is the author of Miracle Stem Cell Heart Repair and Hidden Causes of Heart Attack & Stroke. Website http://WWW.abigon.com Author available for interviews,

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Stem Cells Research - Stem Cell Heart Repair ... - Abigon

Home About Stem Cell Therapy

The Scottsdale Stem Cell Treatment Center provides stem cell therapy for residents of Scottsdale, Phoenix and the surrounding Arizona communities.

Stem-cell therapy is an intervention strategy that introduces new adult stem cells into damaged tissue in order to treat disease or injury. Many medical researchers believe that stem-cell treatments have the potential to change the face of human disease and alleviate suffering. The ability of stem cells to self-renew and give rise to subsequent generations with variable degrees of differentiation capacities offers significant potential for generation of tissues that can potentially replace diseased and damaged areas in the body, with minimal risk of rejection and side effects.

This section will discuss:

And ask the question:

The Cell Surgical Network and its affiliate treatment centers are not offering stem cell therapy as a cure for any condition, disease, or injury. No statements or implied treatments on this website have been evaluated or approved by the FDA. This website contains no medical advice. All statements and opinions provided by this website are provided for educational and informational purposes only and we do not diagnose or treat via this website or via telephone. The Cell Surgical Network and its affiliate treatment centers are offering patient funded research to provide individual patients with Stromal Vascular Fraction that contains their own autologous stem cells and growth factors and the treatment centers provide surgical procedures only and are not involved in the use or manufacture of any investigational drugs.

The Cell Surgical network does not claim that any applications, or potential applications, using autologous stem cells are approved by the FDA, or are even effective. We do not claim that these treatments work for any listed nor unlisted condition, intended or implied. Its important for potential patients to do their own research based on the options that we present so that one can make an informed decision. Any decision to participate in our patient funded experimental protocols is completely voluntary.

ATTENTION: If you have ANY concern with stromal vascular fraction, any of our products, methods, website, or technique and think we may be violating any U.S. law, pleasecontact usso that we can investigate the matter or concern immediately.

The Scottsdale Stem Cell Treatment Center provides cutting-edge care for patients in Scottsdale, Phoenix and the surrounding Arizona communities with a wide variety of degenerative disorders using adult stem cell regenerative therapy.

Marvin Borsand, D.O., F.A.C.O.S, F.A.A.C.S. is focused on providing you with the most innovative techniques and advanced procedures for harvesting and deploying adult stem cells from your own fat.

Read more:
About Stem Cell Therapy in Scottsdale, Phoenix, AZ

Adipose Stem Cell Therapy

What is an Autologous Adipose Stem Cell Procedure?

A small sample of Adipose tissue (fat) is removed from above the Superior Iliac spine (love handles) or abdomen under a local anesthetic.

Obtaining Adipose-Derived Stem Cells (ADSCs) is much easier and less invasive than performing abone marrowextraction. In addition, adipose tissue contains much larger volumes of mesenchymal stem cells than does bone marrow. We use the patient's own adipose tissue to extract the stem cells. Autologous meansthat the donor and the recipient are the same person.

Benefits of ADSCs: Stem cells play an integral part in wound healing and regeneration of tissue at the cellular level.

The Major Advantages of Adipose Stem Cell Therapy:

Is this procedure a significant improvement on other treatments currently available?

Yes We can now obtain Adult Stem Cells (ASCs) from a fat sample. This in-clinic treatment is completed the same day, and there is no need to ship samples to an outside laboratory and wait days for the cells to be returned for an injection on a second visit. This faster process provides increased stem cell counts, without manipulation.

Is an Autologous Adipose Stem Cell Procedure Safe?

Yes because the adipose tissue is removed from one's own body via sterile technique and remains in a controlled environment there are no problems with cell rejection or disease transmission. The interview, physical, harvesting, and administration of stem cells are all performed in-house under a physicians control.

How do I know if stem cell therapy is right for me?

Discussing treatment options with your physician is an important first step in making a decision regarding stem cell therapy. Potential outcomes, an integrative and comprehensive treatment plan, and financial costs are all factors to consider.

I have heard Stem Cell Treatments are VERY expensive, can I afford this?

Yes you can!

Due to our advanced adult stem cell technology provided in the form of an in house procedure, our Stem Cell Center can now provide this service at a fraction of the cost previously incurred. Even better, its a same day procedure.We offer theentirety of our treatment in Phoenix, Arizona -USA and we have been able to lower our cost to a flat rate of $7,100.00 per treatment (including consultation). Fees are subject to change and some more complex proceduresmay incur additional costs.

Why Choose an Adipose Stem Cell Procedure?

Adipose-derived mesenchymal stem cells areeasier to harvest than bone marrowand can be obtained in much larger quantities. In addition, it is much less painful and involves lower risks.

*There is a much shorter time from extraction to the administration oftreatment.No culturing or manipulation is needed using our procedure, as opposed to a bone marrow extraction which requires days or weeksto reach the necessary therapeutic threshold.

*There are no ethical or moral issues involved in harvesting autologous Adult Stem Cells (ASCs).

Are There Detrimental Side Effects from an Adipose Stem Cell Procedure?

No, the adipose tissue is extracted from the patients own body sono foreign donors are used. This minimizes the potential for immune rejection.Our procedure is performed completely in-house and administered by licensed physicians here in the United States. Please keep in mind that every procedure does have its risks, but we do practice sterile technique which makes the risk of infectionvery low.In fact, we have not had any infections develop in any of the stem cell patients we have treated as we take great care in keeping a sterile environment.

What You Can Expect When Visiting the Stem Cell Rejuvenation Center:

Differences Between An Adipose And A Bone Marrow Procedure:

Autologous Growth Factor Components of PRP:

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About Adipose Stem Cell Therapy

Welcome to the webpage for The Arizona Stem Cell Center.

We are the first and original facility offering autologous stem cell transplants derived from adipose tissue in Arizona.

Our unique and innovative process allows us to extract several million stem cells from a single fat biopsy. Our extraction technique involves minimal handling of the cells and same day transplantation. Using a patient's own tissue as the source for cells minimizes rejection of the transplanted tissues, potentially maximizing the effectiveness of the transplant.

Here at Total Wellness/AZ Stem Cell Center, we have been using the technique of PRP (Platelet Rich Plasma) for the past decade for musculoskeletal injuries, autoimmune conditions like Lupus and Multiple Sclerosis, degenerative conditions like osteoarthritis, Parkinson's Syndrome and ALS (Amyotrophic Lateral Sclerosis) and chronic viral conditions (including Epstein-Barr, Cytomegalovirus and Herpes viruses). This is an incredibly versatile therapy that has its roots in the eclectic European medical armamentarium of the 1930's.

Platelet rich plasma can be employed as a matrix graft, often referred to as an autologous tissue graft. This platelet-rich plasma (PRP) matrix is defined as a "tissue graft incorporating autologous growth factors and/or autologous undifferentiated cells in a cellular matrix where design depends on the receptor site and tissue of regeneration." (Crane D, Everts PAM. Practical Pain Management. 2008; January/February: 12- 26) 2008). We enrich the autologous tissue graft with hyaluronic acid for stem cell transplants.

The hypothesized reason why PRP with hyaluronic acid is so useful in autologous tissue grafts with stem cells is that platelets, a normal blood cell that aids in clotting, contain multiple growth factors that stimulate tissue growth. In particular, PRP stimulates the growth of collagen; the main component of connective tissue such as tendons and cartilage. These growth factors include transforming growth factor-? (TGF-B), fibroblast growth factor, platelet-derived growth factor, epidermal growth factor, connective tissue growth factor, and vascular endothelial growth factor.

These growth factors normally recruit undifferentiated stem cells to the site of injury and stimulate new tissue growth. Another constituent of platelets, stromal cell derived factor I alpha allows the newly recruited cells to adhere to the area. Hyaluronic acid is a nutritionally supportive polysaccharide substrate for stem cells that is found abundantly in embryonic tissue. When stem cells are harvested from the patient's own tissues, PRP helps to activate the stem cells to actively become a desired tissue line and Hyaluronic Acid helps support.

In addition, when used with stem cells harvested from the patient's own tissue, PRP messages the stem cells to multiply quickly. This inflammatory response is a major driver of appropriate healing response.

An important consideration is that PRP needs to be prepared in a way to ensure a maximal amount of platelets along with a high concentration of growth factors. Obviously, the more growth factors that can be delivered to the site of injury, the more likely tissue healing takes place. We have found that creating a matrix of Hyaluronic acid (a base connective tissue material) with the PRP and the addition of other growth factors can tremendously expedite the healing process. We are the only clinic in the world to integrate stem cell transplantation with PRP.

Neither Statements, nor products on this site, have been evaluated nor approved by the FDA. Total Wellness offers autologous stem cell treatments. These are not approved treatments, drugs, new drugs, or investigational drugs. We do not manufacture products. If you have concern with a treatment or product that we perform or produce, and think we may be violating any USA law, please contact us immediately, so that our legal team can investigate the matter or concern. All statements, opinions, and advice provided by this website, via wire, or by educational seminars, is provided for educational information only. We do not diagnose nor treat via this website or phone. We offer the above therapies via a doctor/patient established relationship which requires direct contact with the physician. Again, visitors should be aware that we are not claiming that any applications, or potential applications using these autologous treatments, are approved by the FDA, or are even effective. We do not claim that these treatments work for any listed nor unlisted condition, intended or implied.

Call for an appointment

AZ Stem Cell Center14991 W. Bell Rd. Surprise, AZ 85374 (623) 977-0077

info@azstemcellcenter.com

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The Arizona Stem Cell Center